The iBio Technology is a Unique Plant-Based Platform that Accelerates the Development and Manufacturing of High Levels of Target Protein
iBio scientists engineer vectors containing the target sequence within a viral replicon
The vector is transferred to an Agrobacterium host that efficiently introduces target DNA to the plant cell nucleus.
The viral replicon directs the production of large amounts of target-specific messenger RNA in plant cells, which is translated into protein by the plant’s own machinery for several days.
Once the plants have been infiltrated with agrobacteria, the viral sequences of the launch vectors, along with the cloned target sequences, are massively amplified through the action of virally encoded enzymes.
Translation of these recombinant viral vector mRNAs can result in the accumulation of gram quantities of target protein per kilogram of fresh plant tissue in less than a week.
The plants accumulate high levels of target protein in their leaf and stem tissue.
This expression of recombinant protein is termed “transient” expression because the target gene sequence is expressed as protein for a fixed period of time, and the foreign genes are not incorporated into the plant chromosome to result in the creation of transgenic plants.
The plant biomass is harvested, homogenized, and clarified to produce an extract containing the protein of interest.
Proteins are further purified as required using conventional separation and chromatography steps widely used in the biopharmaceutical industry.
Fast Development and Production
The iBio Technology dramatically speeds up drug development and production.
Due to entire plants not being required to be genetically modified, accelerated production and development is possible. By using non-GMO plants as manufacturing platform, the iBio Technology process eliminates the months or years needed by technologies using mammalian cells to find a high-producing, stable cell clone that can be expanded into the trillions of daughter cells required for manufacturing useful amounts of a drug.
Using The iBio Technology, a Novel Protein Product Can be Produced at Factory Scale in Under A Month
Once a desired gene is cloned into the iBio Technology vectors, it is introduced into the leaves of the plants by automated vacuum infiltration technology. Over the next 4-7 days, special features of the proprietary vectors ensure the gene spreads to every cell in the stems and leaves where the desired protein is expressed at extremely high levels.
Flexible Technology Allows for Agile Production
Since the growth of plants and introduction of the iBio Technology vectors is the same regardless of the desired product, a facility using the iBio Technology system can easily produce multiple proteins in the same facility without physical reconfiguration.